hmgb1 detection elisa kit  (Chondrex Inc)

Journal: Materials Today Bio

Article Title: Immediate tumor killing and long-term anti-tumor immunoreaction induced by Bufalin-loaded phototherapeutic Janus membrane in CRC postoperative therapy

doi: 10.1016/j.mtbio.2026.102824

Figure Lengend Snippet: In vivo study in anti-tumor immune response by analysizing the maturation, activation and infiltration of immune-related cells, as well as the level of immune-related cytokines. (A) The maturation of DCs in lymph nodes of mice; (B) The activation of CD4 + /CD8 + T cells in spleens in of mice; (C) The activation of NK cells from spleens of mice. (D–G) The quantitative analysis of flow cytometry, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 and ∗∗∗∗ p < 0.0001. (H) Immunofluorescence photos of CD4 + and CD8 + T cell infiltrating tumor tissues of mice, bar: 100 μm. (I–K) The quantitative analysis of immune-related cytokines: IL-6, TNF-α and IFN-γ, ∗ p < 0.5, ∗∗ p < 0.01, ∗∗∗ p < 0.001 and ∗∗∗∗ p < 0.0001.

Article Snippet: The IFN-γ and TNF-α detection kit was purchased from Biodragon Biotechnology (Beijing), the IL-6 detection kit was obtained from BOSTER Biotechnology (Wuhan).

Techniques: In Vivo, Activation Assay, Flow Cytometry, Immunofluorescence

Journal: Materials Today Bio

Article Title: Immediate tumor killing and long-term anti-tumor immunoreaction induced by Bufalin-loaded phototherapeutic Janus membrane in CRC postoperative therapy

doi: 10.1016/j.mtbio.2026.102824

Figure Lengend Snippet: In vivo anti-tumor immune mechanisim analysis of BU-CDs CA-HA -loaded Janus membrane under NIR: (A–I) Flow cytometry and quantitative analysis of DCs, CD4 + /CD8 + T cells, NK cells and MDSCs, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; (J) Immunofluorescent staining of CD4 + /CD8 + T cells in tumor tissues to illustrate the enhanced tumor-specific identification and infiltration of T cells, bar: 50 μm; (K–L) Elisa test of proinflammatory cytokin (IL-6, TNF-α and IFN-γ) to assess the inflammatory activity, ∗ p < 0.05, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: The IFN-γ and TNF-α detection kit was purchased from Biodragon Biotechnology (Beijing), the IL-6 detection kit was obtained from BOSTER Biotechnology (Wuhan).

Techniques: In Vivo, Membrane, Flow Cytometry, Staining, Enzyme-linked Immunosorbent Assay, Activity Assay

Journal: Inflammation

Article Title: Harmine Derivatives Alleviate LPS-Induced Acute Lung Injury via Targeting GSK3β

doi: 10.1007/s10753-026-02487-6

Figure Lengend Snippet: Anti-inflammatory activity screening of harmine derivatives. A NO levels and ( C ) corresponding IC₅₀ values in RAW264.7 cell supernatants were assessed by the Griess assay. B TNF-α levels and ( D ) corresponding IC₅₀ values were determined by ELISA. Data are mean ± SD. Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test. #### p < 0.0001 vs. control; ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. LPS. p < 0.05 was considered statistically significant

Article Snippet: The enzyme-linked immunosorbent assay (ELISA) detection kit was obtained from DAKEWE (Shenzhen, China), and the ROS detection kit was sourced from Elabscience (Wuhan, China).

Techniques: Activity Assay, Griess Assay, Enzyme-linked Immunosorbent Assay, Control

Journal: Inflammation

Article Title: Harmine Derivatives Alleviate LPS-Induced Acute Lung Injury via Targeting GSK3β

doi: 10.1007/s10753-026-02487-6

Figure Lengend Snippet: ZLWH-38 alleviates LPS-induced inflammatory responses in vitro. A , B TNF-α and IL-6 levels in RAW264.7 cell supernatants were measured by ELISA. C – F Relative mRNA expression levels of TNF-α, IL-6, IL-1β, and iNOS in RAW264.7 cells were determined by RT-qPCR. Data are mean ± SD. Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test. ### p < 0.001, #### p < 0.0001 vs. control; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. LPS. p < 0.05 was considered statistically significant

Article Snippet: The enzyme-linked immunosorbent assay (ELISA) detection kit was obtained from DAKEWE (Shenzhen, China), and the ROS detection kit was sourced from Elabscience (Wuhan, China).

Techniques: In Vitro, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Control

Journal: Inflammation

Article Title: Harmine Derivatives Alleviate LPS-Induced Acute Lung Injury via Targeting GSK3β

doi: 10.1007/s10753-026-02487-6

Figure Lengend Snippet: ZLWH-38 reduces neutrophil infiltration in BALF of LPS-induced ALI mice. A Percentage of neutrophils in BALF determined by flow cytometry. B Quantification of flow cytometry data. C TNF-α levels in BALF measured by ELISA. Data are mean ± SD. Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test. #### p < 0.0001 vs. control; * p < 0.05, ** p < 0.01 vs. LPS. p < 0.05 was considered statistically significant

Article Snippet: The enzyme-linked immunosorbent assay (ELISA) detection kit was obtained from DAKEWE (Shenzhen, China), and the ROS detection kit was sourced from Elabscience (Wuhan, China).

Techniques: Flow Cytometry, Enzyme-linked Immunosorbent Assay, Control